Reduced representation bisufite sequencing (RRBS) is a next-generation sequencing approach for DNA methylation analysis. It uses an enzyme digestion of genomic DNA to provide a partial genome. From there, a bisulfite treatment deaminates unmethylated cytosine residues to become uracil. Methylated cytosines remain in tact and do not get converted.
Bisulfite Conversion of Cytosine to Uracil
Polymerase chain reaction (PCR) of the converted DNA then amplifies the converted uracils as thymines to differentiation between sites that are unmethylated (C) or methylated (T). After sequencing, this data is mapped to reference genome for comparative analysis of methylation patterns.
These posts are intended to document my workflow as I handle and manipulate genomic data within the Mac OSX terminal window. The data processed here are from the Illumina platform.